General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay, 10-plate Kit. (96-0005E1CP0L)
The InCELL Hunter eXpress HEK 293 BRD4(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD4(1) via detection of BRD4(1) levels. This product uses EFC technology. The cell line included overexpresses BRD4(1) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GRPR Activated GPCR Internalization Assay, 2-Plate Kit. (93-0667E3CP5M)
The PathHunter GRPR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GRPR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human B2M ASY
The R-PLEX Human B2M Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring beta-2 microglobulin (B2M) in human serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity and precision Requiring 50 L or less sample volume it performs up to 80 tests on two plates The kit includes a 96-well plate with capture antibody detection antibody buffers diluent and calibrators The assay time is 3 5 hours with a calibration range of 50-10 000 pg/mL
Revvity Health Sciences Inc HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MKK4 (Ser257) Detection Kit, 10,000 Assay Points
Eagle Biosciences Inc Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Abcam Cholesterol Uptake Assay Kit
Cholesterol Uptake Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Mevastatin, HMG-CoA reductase inhibitor, 10MG
MW 390.5 Da, Purity >98%. Potent HMG-CoA reductase inhibitor. Proapoptotic, able to arrest cells in G₁ by down-regulation of CDK4, CDK6 and cyclin D1 and up-regulation of p21 and p27.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical AconItase asy KIt 96Wel
Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Cayman’s Aconitase Assay provides a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates.
Meso Scale Discovery R-PLEX Human Tenascin C ASY
The R-PLEX Human Tenascin-C Assay from Meso Scale Discovery is an electrochemiluminescent immunoassay designed for the quantitative measurement of Tenascin-C in human serum plasma and cell culture supernatants This assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and rapid detection of Tenascin-C an extracellular matrix glycoprotein implicated in tissue injury and cancer progression With a detection range of 1 56-400 ng/mL and minimal required sample volume this assay allows high-throughput and accurate quantification of Tenascin-C levels from small sample sizes
Cayman Chemical SupRoxID DIsmutase asy KI 480
Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet.
Eurofins Discoverx PathHunter eXpress CCR2 Total GPCR Internalization Assay, 2pl Kit. (93-0679E3CP5M)
The PathHunter CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0593E2CP0L)
The PathHunter EDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of EDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRB and EA-tagged β-Arrestin-2. Activation of EDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human Fas (soluble) Assay, 96-well plate, 0.22 pg/mL LLOD, Dynamic Range: 0.22 - 1600 pg/mL, for serum and plasma, Mouse Monoclonal antibodies, Research use only
The U-PLEX Human Fas (soluble) Assay by MESO SCALE DISCOVERY is a sophisticated immunoassay designed for the quantitative detection of soluble Fas in human serum and plasma. This assay utilizes validated components of the U-PLEX platform, simplifying the development of sensitive assays suitable for future multiplexing within Immuno-Oncology analytes. Intended solely for research purposes, this product ensures reliable results in oncological and immunological applications.
- Designed for quantitative detection of soluble Fas.
- Suitable for serum and plasma samples.
- Dynamic range of 0.22 - 1,600 pg/mL.
- Sensitivity level of 0.22 pg/mL.
- Compatible with multiple instruments.
- Incorporates both capture and detection antibodies from mouse monoclonal sources.
- Part of Immuno-Oncology Group 1 multiplex compatibility.
- Multi-component storage conditions may apply.